|How Multi-Omics Profiling Can Redefine Precision Health and Medicine On-Demand Webinar Learn how coupling routine datasets with newer types can unlock the complexity of systems biology at the individual level.||View webinar|
| Label-free cellular analysis with raman imaging |
Presenter: Matt Meyer, Ph.D, Research Scientist, Thermo Fisher Scientific Raman imaging for life science research offers unique analytical capabilities for cell biology, enabling label-free characterization of biological systems with sub-micron spatial resolution. The ability to visualize living samples spatially and temporally to non-invasively understand molecular composition and dynamics has made Raman imaging a promising tool for cellular analysis. The DXRxi Raman imaging microscope allows the user to study living specimens through chemical imaging of components in their native environment. We will illustrate how this is an effective technique for live human cells, bacteria cells and model organisms. Among the topics to be discussed in this webinar: Collection of Raman images from live cells showing the distribution of biological components including nucleic acids, cytochrome c, lipids and proteins. Examples of how Raman imaging can be used for chemical analysis of live cells, bacteria cells and model organisms.
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| Addressing Compound ID in Untargeted Metabolomics |
Metabolomics, although newer to the omics field, is quickly gaining traction in multiple areas of research, as it leads to a more complete picture of the phenotype.Of the current challenges facing the field of metabolomics, compound identification in untargeted metabolomics remains a major bottleneck.Learn about the resources that address this challenge by filling out the form below.
| Advanced Metabolome Annotation with Orbitrap ID-X Tribrid Mass Spectrometer |
Metabolite annotation is a current bottleneck in the broad implementation of metabolomics, hindering biological interpretation of results. In LC/MS based metabolomics, thousands of features from a single sample are routinely detected. However, not all features represent metabolites of biological origin and fragmentation spectra of background ions and degenerate signals detract from metabolite identification efforts. Thermo Fisher Scientific is addressing the identity crisis with novel workflow strategies for data acquisition and analysis. The AcquireX intelligent acquisition on the Orbitrap ID-X Tribrid MS automatically minimizes the fragmentation of background ions and degenerate signals, and efficiently generates more spectral information on relevant compounds. Advanced annotation tools like mzLogic in the Compound Discoverer software take advantage of information-rich data combined with the vast chemical knowledge of the mzCloud library increasing confidence in unknown annotation.
| On-Demand Webinar – Escape the Metabolomics Identity Crisis with Intelligence Driven Mass Spectrometry |
Small molecule unknown identification is challenging due to the vastly diverse chemical space with exponential possibilities for any given compound. High-resolution accurate-mass spectrometry delivers informative analytical measurements providing biological insight. Yet, converting mass spectra into confidently annotated compounds is a difficult process due to the complexity of small molecules hindering confident biological interpretation.
| A New Strategy Optimized for Metabolite Profiling on Thermo Scientific Orbitrap ID-X Tribrid Mass Spectrometer Platform |
A new data-dependent acquisition workflow is developed by using real-time background subtraction on a Thermo Scientific Orbitrap ID-X Tribrid mass spectrometer with AcquireX intelligent data acquisition strategy. This workflow enables collecting a complete dataset of high-resolution full MS and MSn scans of all potential metabolites in automated fashion, which needs neither pre-existing knowledge nor building exclusion and inclusion lists manually. Customized data-mining software is used for in-depth metabolite identification and structural elucidation. The results demonstrate that the Orbitrap ID-X MS with AcquireX data acquisition strategy coupled with advanced data processing software can improve metabolite analysis efficiency and quality.
| New Generation of Software Tools for the Identification of Compounds |
Addressing the “Identity Crisis” in Small Molecule Compound Identification isn’t easy. Determining the chemical structure of the majority of compounds “identified” in a typical untargeted analysis remains a stubborn challenge and a persistent logjam, holding up important progress in many fields including metabolomics, metabolism studies, contaminant analysis, and extractables & leachables identification.
|Features or Compounds? A Data Reduction Strategy for Untargeted Metabolomics Analyses to Generate Meaningful Data||View webinar|
| New Developments for Compound Annotation and Identification in Metabolomics Webinar On Demand Webinar |
Learn about untargeted metabolomics challenges for compound annotation and ID using new and advanced MS workflows.
| Metabolomic Data Analysis: Solutions and Challenges Ahead |
Metabolomics presents unique challenges in terms of data acquisition, data processing, data standardization, statistical analyses, and identification of unknowns. Robust and precise analytical methods are essential to metabolomics, but data acquisition is only part of the challenge. It is equally important to develop both software tools and appropriate workflows to extract meaningful data and generate results from metabolomics datasets.
This webinar provides an overview of approaches and software post-data acquisition workflows, including novel tools being used in the detection of statistically significant patterns and identification of underlying compounds and their pathway context. Finally, current approaches in integrating metabolomic data with other multi-omic datasets will also be explored.
| Credentialing Features to Benchmark Metabolomic Methods and Technologies |
The aim of untargeted metabolomics is to comprehensively profile the full range of metabolites present within a biological sample. Several experimental factors strongly influence success: extraction method, separation strategy, ionization mechanism, mass spectrometer, and informatic software. Which combination of technologies is best? Although counting signals (i.e., metabolomic features) has commonly been used as a standard metric of comparison, this approach can be misleading and provide inaccurate results due to the significant number of artifacts in metabolomic data sets.
Here we present an alternative strategy for benchmarking metabolomic technologies called credentialing. The credentialing approach facilitates removal of artifactual features without the resource-intensive burden of structural identification. Some surprising insights gained from the application of credentialing will be presented.
| You Are What You Eat: Metabolomics Analysis of Alternative Diets |
Learn about method parameters, the resulting diverse data sets and the definitive tools used for unambiguous compound identification that promise to bring metabolomics and lipidomics efforts to an entirely new level. This webinar showcases a workflow incorporating various analyses – starting with the Sample – Sample Prep – Chromatography – Mass Spectrometry – Data Review and Analysis – and Integration into the Cloud
| The Promise of Mass Spectrometry-Based Metabolomics for Clinical Research |
In this webinar, Dr. Rick Dunn discusses how and why metabolomics is applied in clinical research, how mass spectrometry can be applied to acquire robust data on thousands of metabolites in small and large-scale studies of the human population and talks in length about the first-example of large-scale untargeted metabolic profiling applying UHPLC-MS, called the HUSERMET project.
| Investigating Time of Death Using a Novel, High Resolution Orbitrap-based GC-MS/MS System |
In this webinar Karl Burgess, a lead scientist in metabolomics research from Glasgow Polyomics and Cristian Cojocariu, senior application scientist at Thermo Fisher Scientific, talk about solutions to some of the analytical challenges in metabolomics research related to the new Thermo Scientific Q Exactive GC Hybrid Quadrupole-Orbitrap GC-MS/MS System. Specifically, Dr. Burgess presents results on his laboratory’s studies using the Q Exactive GC Orbitrap GC-MS/MS system to study the metabolome as a means to determine post-mortem interval.
|LC/MS-Based Metabolite Profiling and Its Applications in Basic Biomedical Research||View webinar|
| Non-targeted and Targeted Analysis of Anionic Metabolites Using Ion Chormatography and High Resolution Accurate Mass Spectrometry |
Non-targeted and Targeted Analysis of Anionic Metabolites Using Ion Chormatography and High Resolution Accurate Mass Spectrometry
|Effective Use of Novel Data Independent Analysis Methods to Obtain Comprehensive and Reproducible Characterization of a Biological Pathway||View webinar|
|High Resolution Mass Spectrometry Strategies for an Integrated Metabolomics||View webinar|
|Metabolic Profiling of Anionic Metabolites in Head and Neck Cancer Cells by Capillary Ion Chromatography with Orbitrap Mass Spectrometry||View webinar|
| Enhancing Productivity and Accuracy in Drug Metabolism Studies with the Latest Orbitrap Technology |
This webinar explores how productivity and accuracy in drug metabolism studies can be improved by employing the latest advancement in Orbitrap technology in pharmaceutical science and related industries.
| Mass Spectrometry in Proteomics |
Join thought leaders and peers at the proteomicsNOW virtual conference to learn about the latest advances in proteomics, share best practices, and explore new technologies and techniques designed to help improve your research results.This event highlights recent developments in proteomics through live webinars, lightning talks, poster sessions, and panel discussions. Since we know how hard it is to set aside long periods of time to watch groundbreaking scientific talks, the event is available on demand as well.
| One Cell at a Time: Single Cell Proteomics Becomes a Reality |
Mass spectrometry (MS) workflows are poised to overcome limitations in our ability to identify and quantify proteins in single cells. In this webinar, Dr. Ryan Kelly will describe: nanoPOTS, a new sample processing platform to maximize sample recovery; miniaturization of LC separations; and latest-generation of MS instrumentation to provide the required sensitivity and throughput for effective analysis of such trace samples.
| Multiplexing Single Cell Proteomics: A Marriage of Sensitivity and Throughput |
In this webinar, Dr. Erwin Schoof will describe how a 384-well workflow, using standard off-the-shelf components, allows characterization of single cells to a protein depth of well over 1,000 proteins per cell. Dr. Schoof will introduce the latest generation HRAM Thermo Scientific™ Tribrid™ MS instrumentation with FAIMS technology for single cell-level sensitivity. When combined with new Thermo Scientific™ TMTPro reagents and optimized chromatography, researchers can analyze >100 single cells per day per instrument. An in-house developed computational pipeline, SCeptre, helps derive biological meaning from the data.
| Quantitative approaches in discovery proteomics: How do they measure up? |
Mass spectrometry-based proteomics offers unbiased, systems-level insights to biological systems. Proteomics has the potential to characterize the structure, function, and activity of protein components at unparalleled depth. Quantitative discovery approaches allow not only the identification of various protein components within these systems but also their abundance levels, or expression status. A variety of techniques and methodologies are employed to survey and quantify proteomes in discovery mode.
| Improving Proteome Coverage and Peptide Identification Rates in Short LC Gradients |
Keeping abreast of the ability to increase proteome coverage with advances in instrumentation and workflows is of great interest to proteomics investigators.In this webcast, the speaker will present first impressions of the Thermo Scientific Orbitrap Exploris 480 mass spectrometer equipped with a Thermo Scientific FAIMS Pro interface for shotgun proteomics. The performance of the new instrument is evaluated in data-dependent acquisition (DDA) and data-independent acquisition (DIA)* modes with fast scanning HCD methods in combination with front-end Field Asymmetric Ion Mobility Spectrometry (FAIMS).
| Achieve High Throughput LC-MS Screening while Maintaining Protein Depth and Quantitative Accuracy using an Intelligence-Driven MS and Differential Ion Mobility |
For proteomics laboratories who want to obtain better quality data for discovery of disease biomarkers or identification of new therapeutic targets, the FAIMS Pro interface provides increased selectivity. In this on-demand webinar, learn about how the combination of the Thermo Scientific Orbitrap Exploris 480 mass spectrometer with the FAIMS Pro interface significantly increased proteome coverage when using single VCs and short LC gradients.
| Instant Answer: All the Data in Half the Time Using a Real-time Database Search for Isobaric Labeling Studies |
Quantitative proteomics strategies using Tandem Mass Tags (TMT) enable precise measurement of protein abundance from multiple samples into a single high-resolution LC/MS analysis. Increasingly, various biological experiments demand higher multiplexing and sample throughput with accurate quantitation. This webinar presents strategies that combine next generation technologies in multiplexing chemistry, hardware and software for increased quantitative performance and throughput efficiency on Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer. This includes Advanced SPS-MS3 approaches using next generation isobaric tags and real-time search. Together, these advances allow for intelligent acquisition methods that improve quantitation accuracy, precision and proteome depth.
| Enhanced Proteome Analysis With FAIMS-MS |
In this session, you will learn why ion mobility isn’t what you’ve been missing for high throughput proteomics. Learn how the Thermo Scientific FAIMS Pro interface when connected with Thermo Scientific LCMS systems enables Dr. Westphall to obtain up to a 20% or more increase in the number of proteins and peptides his lab can detect in a single experiment; i.e. a single elution profile from an LC. You will learn how traditional ion mobility has limited utility when looking at cross-sections and other information. He describes how the gas phase separation technique helps immensely along with added benefits of the ease-of-use and reduction in cleaning their LCMS.
| Improvement of sensitivity and comprehensiveness of proteomic analyses using a novel FAIMS Pro Interface |
Discovery proteomics, translational research, and precision medicine perform global proteome profiling of large sample sets to identify putative protein and/or peptide biomarkers across different biological conditions. In this webinar Dr. Pierre Thibault will present a simple, new step to their existing workflow with the addition of a novel gas phase ion fractionation device to increase proteome coverage and confidence in global proteome profiling. This study will highlight the advantages of the Thermo Scientific FAIMS Pro device in quantitative proteomics using SILAC and TMT labelling to improve the accuracy, precision, and comprehensiveness of quantitative measurements.
| Ultrahigh Performance for Resolving Proteoforms in Targeted and Discovery Modes |
Laboratories who are looking to obtain rich information from proteoforms will benefit from this on-demand webinar about high quality datasets generated from the Thermo Scientific Orbitrap Eclipse Tribrid MS platform. The presented data will cover both denatured and native modes for the analysis of whole proteoforms and their complexes. This involves fast LC-MS/MS runs for denatured mode analysis of proteoforms in targeted or complex mixtures, and very high dynamic range for analysis of antibodies and protein complexes up to 8000 m/z using native top-down mass spectrometry.
| How to Maximize Label-Free Quantitation (LFQ) on a Tribrid Mass Spectrometer |
Translational and Clinical researchers are challenged with the growing complexities of qualitative and quantitative analyses in proteomics. The Orbitrap Tribrid mass spectrometers provide substantial advantages to improve label-free quantitation (LFQ) performance due to its unique architecture and capabilities. The Tribrid architecture facilitates novel data acquisition methods to surpass the primary challenges associated with successfully acquiring high-quality data that enables confident, automated data processing to more accurately determine the expression ratios for peptides, and more importantly, proteins, in complex biological samples.
| Digging Deeper in Every Direction: Developing a Next-Generation Platform for High-Throughput Multi-Faceted Protein Characterization |
Comprehensive LC-MS-based characterization of therapeutic proteins requires detailed molecular knowledge of every residue and sidechain, as well as every intact protein isoform.With the goal of maximizing molecular understanding per unit time we will discuss a platform for addressing the versatility and throughput requirements of performing comprehensive characterization. We will focus on applying advanced versions of multiple key characterization workflows using a battery of state-of-the-art LC-MS methods performed on a single Orbitrap mass spectrometer.
| Emergence of Clinical Proteomics: Identifying and developing actionable protein biomarkers for use in clinical research |
This webinar focuses on development and validation of proteomics-based oncology biomarkers using peptide surrogates. A descriptive overview is provided for the assay development pipeline utilized to bring proteomics markers from targeted-discovery experiments to multiplexed SRM methods, with validated methods translated into the CAP-CLIA lab.
| Structural Analysis of Ribosome Particles by Mass Spectrometry Toolbox for Structural Biology |
To understand protein function and mechanism of action, it is essential to determine protein complex assembly and structure. Thermo Fisher Scientific leads the way in accelerating protein structure-function studies with its Integrative Structural Biology solutions. New Thermo Scientific™ Orbitrap-based mass spectrometers enable characterization of megadalton complexes and dynamic structure-function relationships. In this symposia, you will learn about new approaches for structural proteomics workflows for crosslinking and native MS.
| MS Toolbox for Structural Biology |
To understand protein function and mechanism of action, it is essential to determine protein complex assembly and structure. Thermo Fisher Scientific leads the way in accelerating protein structure-function studies with its Integrative Structural Biology solutions. New Thermo Scientific Orbitrap-based mass spectrometers enable characterization of megadalton complexes and dynamic structure-function relationships. In this workshop, our expert speaker, Dr. Rosa Viner will share new approaches for structural proteomics workflows for crosslinking and native MS.
| Structural Tools In Your MS Tool Box |
Integrative methods in structural biology can generate molecular models for protein assemblies at any scale. The approach begins by levering existing structural knowledge as “building blocks”. It then incorporates different sources of biophysical data to fill in the gaps, armed with powerful computational routines.Top of Form
| Advancing Mass Spectrometry-Based Large-Cohort Proteomics for Precision Medicine On-Demand Webinar |
Learn about the study of an analytical workflow using HRMS1-DIA mass spectrometry systematically applied to multiple Cancer Moonshot sites.
| 2016 iQuan Series: High Resolution Accurate Mass Peptide Quantitation on Thermo Scientific™ Q Exactive™ Mass Spectrometers |
Describes workflows and step by step process for high resolution accurate mass (HRAM) based peptide quantitation.
| 2016 iQuan Series: Best Practices For Peptide Quantitation On a Triple Quadrupole Mass Spectrometer |
Describes workflows and step by step process for peptide quantitation using triple quadrupole mass spectrometer.
| Translational proteomics: From one-by-one to all-in-one Clinical Assays |
Mass spectrometry plays a key role in the translational proteomics pipeline: whereas continuous improvements in technologies at the discovery side increase qualitative and quantitative coverage of the entire proteoform diversity, ongoing optimization of methodologies at the clinical diagnostic site improve analytical quality and standardization. Integrating both ends would streamline the discovery of new biomarkers by increased analytical quality, and revolutionize clinical diagnostic assays by adoption of multi-protein strategies, including isoforms and post-translational modifications. In this presentation Irene van den Broek, post-doctoral researcher at the Advanced Clinical Biosystems Research Institute at Cedars-Sinai Medical Center will show both the potential of multiplexed multiple reaction monitoring mass spectrometry to fulfill the quality requirements for clinical application as well as discuss challenges and potential strategies for clinical application of data-independent acquisition mass spectrometry as a tool to quantify and identify myofilament proteoforms in heart biopsies.
| Simplifying Complexity: New MS Reagents that Improve Proteome Depth, Quantification, and Throughput |
In this webinar, Dr. John Rogers discusses an optimized proteomics sample preparation workflow for isobaric tags and highlights new tools for fractionation, sample throughput, accurate quantitation that enable researchers to answer complex biological questions. In particular, new high pH reversed phase spin columns which reproducibly and effectively fractionate peptide samples, resulting in a >100% increase in the number of unique peptides and >50% increase in unique protein identifications, as well as multiplexing strategies with TMT10plex to improve sample throughput and reduce overall analysis time will be highlighted.
| Multiplexed Quantitative Proteome and Ubiquitylome Analysis Utilizing nLC-MS/MS and Tandem Mass Tags |
Today, by utilizing isobaric labels (e.g., Tandem Mass Tags), >8,000 proteins can be routinely quantified across 10 experimental samples in 1.5 days of analysis. In this webinar both sample preparation and instrument methods used to collect large scale quantitative proteomic data will be presented by Christopher Rose. The MS3 instrument method (i.e., synchronous precursor selection MS3, SPS-MS3) that removes precursor interference and alleviates compression of measured ratios will also be highlighted.
|Data-Independent Analysis and Targeted MS/MS Quantification of Biomarkers||View webinar|
|Global Protein Profiling Using pSMART on the Q Exactive Mass Spectrometer: Integrated LC-MS Strategies for Comprehensive Profiling and Differential Quantitative Analysis||View webinar|
|Multiplexing Mass Spectrometry-Based Identification and Quantification of Site-Specific Reversible Cysteine Modifications||View webinar|
|High Resolution Native Mass Spectrometry in Biopharmaceuticals: From Protein / Ligand Screening to Therapeutic Protein Characterization||View webinar|
| Orbitrap Hits the Sweet Spot: Thermo Fisher Scientific Solutions For Glycan Analysis |
Mass spectrometry has emerged as one of the most powerful tools for the structural elucidation of glycans. Due to its sensitivity of detection and its ability to analyze complex mixtures of glycans, MS based methods is the maximization of structural information relative to the amount of time, labor and sample quantity.
|Characterization of Glycoproteins: 25 Years of HPAEC-PAD Analysis||View webinar|
|The Sweet Revolution in Mass Spectrometry Based Characterization of Glycans||View webinar|
|The Sweet Revolution in Glycans and Antibody Separations||View webinar|
|Oligosaccharide Analysis by High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection||View webinar|
|LipidSearch – A New Solution for Untargeted Lipidomics Using High-Resolution Accurate Mass LC/MS-MS||View webinar|
|Comprehensive Shotgun Lipidome Analysis Using Novel Sample Preparation Strategies and High Resolution / Accurate Mass Spectrometry||View webinar|
|Shotgun Lipidomics at High Mass Resolution||View webinar|