Implementation of real-time update for time scheduled targeted peptide quantification (PRM) on a new quadrupole Orbitrap benchtop mass spectrometer

In targeted peptide quantification, it is necessary to cover hundreds of targets with high absolute sensitivity at the early stages of the biomarker development pipeline. This is commonly achieved by combining nano-LC with time-scheduled retention time (RT) lists. To maximize the number of scheduled targets and enable their detection with high sensitivity, the number of overlapping RT windows has to be minimized by keeping the windows as narrow as possible. However, this is rendered difficult by the relatively high retention time variations of nano-LC systems.

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