IC: Eluent Generation for Oligo-, Polysaccharide and Aminoglycosides

Let’s start with an innovation that has the potential of changing the landscape of HPAEC-PAD. As you know, carbohydrates can be separated at high pH using hydroxide based eluents. Until very recently, we had to refer to “manually prepared eluents”, when it came to gradient elution needed for oligo- and polysaccharides. It was the acetate, needed as “pushing ion”, that forced us to rely on manual eluent preparation, rather than applying an automated inline eluent generation based on RFIC (Reagent-Free Ion Chromatography). So far, we were able to produce pure hydroxide eluents, methanesulfonic acid (MSA) eluents, and carbonate based eluents using our Eluent Generators. The brilliant idea our colleagues had is to use methanesulfonate as the pushing ion, and thus combine a hydroxide and an MSA eluent generator in series (Dual EGC Mode). This idea was described by our researchers in a White Paper some time ago, and today it can be used with our new ICS-6000 instrument. Please find the first application note using this approach and an in-depth description of the approach here: AN 72714. RFIC for carbohydrate separations – how cool is that? Just think about the benefit of not having to prepare hydroxide and acetate eluents manually anymore.
Talking about carbohydrate separations and RFIC:
In a recently published application note (AN 72720) Sachin and Jeff address the critical question of sample preparation for the accurate determination of the monosaccharide moiety of a glycoprotein. They demonstrate the use of a commercially available glycopeptide standard as a control to determine glycoprotein hydrolysis reaction efficiency. They use the Dionex CarboPac PA20-Fast-4µm in conjunction with electrolytically generated KOH-eluents and pulsed amperometric detection. Quite interesting read, I think.
Now let’s talk about aminoglycoside determinations. One of the several approaches available for aminoglycoside (AmG) determination is ion-pair chromatography, which is accepted by both EP and USP. In the two following application notes (AN 72647, AB 72648) our colleagues describe the use of a for the separation of AmGs specially designed reversed phase column (Dionex IonPac Dionex IonPac AmG-3µm C18) in conjunction with pulsed amperometric detection. While the first is following the official guidelines of USP and EP, the second paper shows a way how to simplify the eluents used for the separation on our modular ICS-systems.

While you and I are familiar with IC, from time to time, I’d like to have some additional background information on MS. And that is the reason, why I would like to share a White Paper published by our colleague Debadeep Bhattacharyya talking about triple-quadrupole MS and Orbitrap-HRAMS, their similarities, differences, and capabilities. I truly enjoyed reading, and hope you will find it as nicely written, and informative as I did.

I hope you will enjoy the read and find this information contained interesting.

Source:

Dr. Detlef Jensen
EU Sales Support Expert for
Ion Chromatography and Environmental Market

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