New biopharma applications and upcoming events

Autumn, 2018

Autumn is here, and so are our latest protein characterization applications, and a workshop and webinar to look out for.

 

Biopharma Characterization Workshop – Reinach
November 29, 2018

Learn about biopharma characterization workflows from Thermo Scientific experts
Observe live demonstration and evaluation of innovative automatable sample preparation solutions, software solutions for peptide and intact protein analysis workflows
Learn about Instrumentation and Software built for BioPharma: Vanquish UHPLC and Q Exactive HRAM mass spectrometers combined with Chromeleon CDS, the software to rule them all

See the agenda »

 

Webinar: Multi Attribute Method and Native Intact Mass Spectrometry for Characterization of Quality Attributes During Pharmaceutical Development of mAb Mixtures
December 5, 2018: 9 a.m. PST | 12 p.m. EST | 14:00 CET
Join Dan Bach Kristensen, Principal Scientist for this webinar and learn how experts at Symphogen are looking increasingly to mass spectrometry for qualitative and quantitative control of critical quality attributes (CQAs) of anti-cancer mAb mixtures during biopharmaceutical development.

Learn how peptide mapping using high resolution accurate mass (HRAM) mass spectrometry provides the necessary chromatographic and mass spectral resolution to allow control of critical quality attributes (CQAs) in complex mAb mixtures
Learn how the same high resolution MS Platform can be used for peptide mapping and native mass spectrometry characterization of biopharmaceuticals

Register here »

 

Comparability study for the determination of post-translational modifications of infliximab innovator and biosimilars
New application note created by NIBRT, Ireland:

A study to evaluate automated peptide mapping by combining the use of the Thermo Scientific™ SMART Digest™ Trypsin Kit, with Magnetic Bulk Resin option (Magnetic SMART Digest), with the Thermo Scientific™ KingFisher™ Duo Prime purification system to investigate PTMs in the innovator and biosimilar drug substances.
The efficiency and reproducibility of the platform was evaluated with a specific focus on the determination of protein sequence coverage and identification of post-translational modifications (PTMs), including deamidation, oxidation, lysine clipping, glycation, and glycosylation.

Application notes »

 

More about peptide mapping »

 

Comparability study for the determination of post-translational modifications of biotherapeutics and biosimilars
New application note created by NIBRT, Ireland:

Peptide mapping study to evaluate the similarity of post-translational modifications (PTMs) detected in Rituximab and Trastuzumab drug products and their respective biosimilars.
Rapid, automated sample preparation within one hour leading to highly reproducible results for innovator and biosimilar comparability studies, with less hands-on time.
Simple protein digestion with minimal user intervention while achieving high confidence in results with excellent data quality; approximately 100% sequence coverage and low levels of sample preparation induced post-translational modifications.

Application note »

 

More about SMART Digest Kits »

 

Salt gradient analysis of IgG1 monoclonal antibodies using a 5 µm WCX chromatography column

Learn about high-resolution separation of IgG1 therapeutic mAb charge variants using a salt gradient with the Thermo Scientific™ ProPac™ Elite WCX 5 µm columns
See how intuitive method development may be performed
Deliver fast analysis times with optimized gradients

Application note »

 

More about ProPac Elite WCX Columns »

 

NISTmAb characterization with a high-performance RP chromatography column:

Learn how to achieve intra- and inter-column reproducibility in retention time with overall run-to-run %RSD at 0.12% and column-to-column %RSD at 0.33%
Proven column robustness with minimal column degradation after more than 1000 monoclonal antibody injections
See how consistently low carry-over with up to 96.4% column recovery was achieved for accurate quantification
Learn how high accuracy and precision was achieved at all different QC levels within 93–104% accuracy

Application note »

 

More about MabPac RP Columns »

 

SMART Digest Peptide Mapping and Quantitation Compendium
Peptide Mapping and Quantitation – Easier, Faster and More Reproducible.
Learn how SMART Digest can be leveraged to perform:

Faster and more sensitive protein characterization and quantitation
Easier digestion
Faster digestion
Highly reproducible digestion
Automation of digestion

Download compendium »

 

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